Development of a Remotely-Piloted Vehicle Platform to Support Implementation, Verification, and Validation of Pilot Control Systems

This thesis presents the development of a research test bed and the use of a set of metrics for evaluating handling qualities with pilot in the loop configuration. The main objective of this study is to provide software and hardware tools to support performance evaluation of control systems designed to compensate for Pilot Induced Oscillations (PIOs). A remotely-piloted vehicle presented in this thesis consists of an RC aircraft modified to be flown from a ground station cockpit. The unmanned aerial system has a high-speed on-board processing system capable of simulating different conditions during flight such as injecting actuator failures and adding delays. In this study, the analysis of pilot handling qualities based on a set of evaluation metrics, is also included. The metrics are based on time-domain Neal-Smith criterion and are used to provide numerical data which categorizes the control system in one of the levels on the Cooper-Harper Rating scale. Two different control configurations were implemented and analyzed in this study: stick-to-servo and non-linear dynamic inversion control laws. Piloted-simulation results are presented on the Neal-Smith flying qualities plane at different flight conditions

Nebulized mesenchymal stem cell derived conditioned medium ameliorates Escherichia coli induced pneumonia in a rat model

BackgroundMesenchymal stem cells (MSC) have shown immense therapeutic promise in a range of inflammatory diseases, including acute respiratory distress syndrome (ARDS), and are rapidly advancing through clinical trials. Among their multimodal mechanisms of action, MSCs exert strong immunomodulatory effects via their secretome, which contains cytokines, small molecules, extracellular vesicles, and a range of other factors. Recent studies have shown that the MSC secretome can recapitulate many of the beneficial effects of the MSC itself. We aimed to determine the therapeutic capacity of the MSC secretome in a rat bacterial pneumonia model, especially when delivered directly to the lung by nebulization which is a technique more appropriate for the ventilated patient.MethodsConditioned medium (CM) was generated from human bone marrow derived MSCs in the absence of antibiotics and serum supplements. Post-nebulization lung penetration was estimated through nebulization of CM to a cascade impactor and simulated lung and quantification of collected total protein and IL-8 cytokine. Control and nebulized CM was added to a variety of lung cell culture models and injury resolution assessed. In a rat E. coli pneumonia model, CM was instilled or administered by nebulization and lung injury and inflammation assessed at 48 h.ResultsMSC-CM was predicted to have good distal lung penetration and delivery when administered by nebulizer. Both control and nebulized CM reduced NF-κB activation and inflammatory cytokine production in lung cell culture, while promoting cell viability and would closure in oxidative stress and scratch wound models. In a rat bacterial pneumonia model, both instilled and nebulizer delivered CM improved lung function, increasing blood oxygenation and reducing carbon dioxide levels compared to unconditioned medium controls. A reduction in bacterial load was also observed in both treatment groups. Inflammatory cytokines were reduced significantly by both liquid and aerosol CM administration, with less IL-1β, IL-6, and CINC1 in these groups compared to controls.ConclusionMSC-CM is a potential therapeutic for pneumonia ARDS, and administration is compatible with vibrating mesh nebulization

Editorial introduction

This article frames the journal special issue by offering a broad reflection on the historical development of ideas that have informed debates concerning intermediality and its pedagogical contexts. It opens with a brief articulation of media and intermedial theory to inform the debate. The challenges of contemporary media hybridity are then set within an historical context by tracing the origins of current (perceived) knowledge dichotomies and hierarchies into the philosophical canons of western antiquity. In examining distinctions between the different types of knowledge and expression that form the constituent parts of contemporary intermedial theatres, the article considers philosophical debates, traces historical trajectories and probes social dynamics from Aristotle to the present. Moving on to the current historical and social context of intermedial practice and pedagogy, the article examines specific challenges and opportunities that emerge from our own intermedial age. This multifaceted and trans-historical approach leads the authors to suggest that old hierarchical and divisional structures impact upon contemporary practices, affecting how those are perceived, received and valued

Multiple Dosing and Preactivation of Mesenchymal Stromal Cells Enhance Efficacy in Established Pneumonia Induced by Antimicrobial-Resistant <i>Klebsiella pneumoniae</i> in Rodents

Antimicrobial-resistant (AMR) bacteria, such as Klebsiella species, are an increasingly common cause of hospital-acquired pneumonia, resulting in high mortality and morbidity. Harnessing the host immune response to AMR bacterial infection using mesenchymal stem cells (MSCs) is a promising approach to bypass bacterial AMR mechanisms. The administration of single doses of naïve MSCs to ARDS clinical trial patient cohorts has been shown to be safe, although efficacy is unclear. The study tested whether repeated MSC dosing and/or preactivation, would attenuate AMR Klebsiella pneumonia-induced established pneumonia. Rat models of established K. pneumoniae-induced pneumonia were randomised to receive intravenous naïve or cytomix-preactivated umbilical cord MSCs as a single dose at 24 h post pneumonia induction with or without a subsequent dose at 48 h. Physiological indices, bronchoalveolar lavage (BAL), and tissues were obtained at 72 h post pneumonia induction. A single dose of naïve MSCs was largely ineffective, whereas two doses of MSCs were effective in attenuating Klebsiella pneumosepsis, improving lung compliance and oxygenation, while reducing bacteria and injury in the lung. Cytomix-preactivated MSCs were superior to naïve MSCs. BAL neutrophil counts and activation were reduced, and apoptosis increased. MSC therapy reduced cytotoxic BAL T cells, and increased CD4+/CD8+ ratios. Systemically, granulocytes, classical monocytes, and the CD4+/CD8+ ratio were reduced, and nonclassical monocytes were increased. Repeated doses of MSCs—particularly preactivated MSCs—enhance their therapeutic potential in a clinically relevant model of established AMR K. pneumoniae-induced pneumosepsis

Aversion to Injection Limits Acceptability of Extended-Release Naltrexone Among Homeless, Alcohol-Dependent Patients

BACKGROUND: Ending homelessness is a major priority of the Department of Veteran Affairs (VA), and alcohol use can be a barrier to stable housing. Clinical trials suggest that depot extended-release naltrexone (XR-NTX) is efficacious in reducing alcohol consumption among alcohol-dependent subjects. METHODS: An open-label, randomized pilot study sought to examine the feasibility and effectiveness of XR-NTX versus oral naltrexone to improve alcohol consumption and housing stability among homeless, alcohol-dependent veterans at the Providence Veteran Affairs Medical Center. RESULTS: Of 215 potential candidates approached over a 16-month recruitment period, only 15 agreed to consider study entry and 7 were randomized. The primary reasons given for refusal were not wanting an injection; fear of needles; and not wanting to change drinking habits. Only 1 participant in the XR-NTX group returned after the first injection. Three participants in the oral naltrexone group attended all 7 visits and had good outcomes. CONCLUSIONS: Although XR-NTX has demonstrated efficacy in reducing heavy drinking, limited acceptance of the injection might reduce its effectiveness among homeless, alcohol-dependent patients

Student Recital (April 28, 2016)

Sonata / Leo Brouwer Sarabanda de Scriabin Sean Donovan, guitar Man is for the Woman made / Henry Purcell Briana Crockett, mezzo-soprano Sonatina für Posaune und Klavier / Kazimierz Serocki Allegro Andante molto sostenuto Nicholas Grisolia, euphonium Hymn / Domenic Argento Cinq mélodies / Gabriel Fauré Mandoline Angela Maloney, soprano Ständchen, D.957 / Franz Schubert Patrick Dauwer, baritone Concertino for Trombone and Wind Orchestra / Gordon Jacob Cody O’Toole, trombone Zuiegnung / Richard Strauss Justine Smigel, mezzo-soprano Sonata for Flute and Piano / Paul Hinemith Sehr lebhaft Marsch Ian Maguire, flute Ich folge dir glecihfalls (Johannes-Passion) / Johann Sebastian Bach Waiting since February (Er Yue Li Jian Ba Dao Ru Jin) / Shanbei Folk Song, arr. Yin Xiang Jiaying Zhu, soprano Ian Maguire, flute Il segreto per esser felici (Lucrezia Borgia) / Gaetano Donizetti Quatre mélodies, Opus 39 / G. Fauré Les Roses d’Ispahan Marie Doyon, mezzo-soprano Trumpet Concerto in Eb Major, Hob. Vlle/1 / Franz Joseph Haydn Allegro Tristen Fuller, trumpethttps://vc.bridgew.edu/student_concerts/1115/thumbnail.jp

Differential Effects of Cytokine Versus Hypoxic Preconditioning of Human Mesenchymal Stromal Cells in Pulmonary Sepsis Induced by Antimicrobial-Resistant Klebsiella pneumoniae

Background: Pulmonary sepsis is a leading cause of hospital mortality, and sepses arising from antimicrobial-resistant (AMR) bacterial strains are particularly difficult to treat. Here we investigated the potential of mesenchymal stromal cells (MSCs) to combat established Klebsiella pneumoniae pneumosepsis and further evaluated MSC preconditioning and pre-activation methods. Methods: The potential for na&iuml;ve and preconditioned MSCs to enhance wound healing, reduce inflammation, preserve metabolic activity, and enhance bacterial killing was assessed in vitro. Rats were subjected to intratracheal K. pneumoniae followed by the intravenous administration of MSCs. Physiological indices, blood, bronchoalveolar lavage (BAL), and tissues were obtained 72 h later. Results: In vitro assays confirmed that preconditioning enhances MSC function, accelerating pulmonary epithelial wound closure, reducing inflammation, attenuating cell death, and increasing bacterial killing. Cytomix-pre-activated MSCs are superior to na&iuml;ve and hypoxia-exposed MSCs in attenuating Klebsiella pneumosepsis, improving lung compliance and oxygenation, reducing bacteria, and attenuating histologic injuries in lungs. BAL inflammatory cytokines were reduced, correlating with decreases in polymorphonuclear (PMN) cells. MSCs increased PMN apoptosis and the CD4:CD8 ratio in BAL. Systemically, granulocytes, classical monocytes, and the CD4:CD8 ratio were reduced, and nonclassical monocytes were increased. Conclusions: Preconditioning with cytokines, but not hypoxia, enhances the therapeutic potential of MSCs in clinically relevant models of K. pneumoniae-induced pneumosepsis

Phenotypic Variation of Helicobacter pylori Isolates from Geographically Distinct Regions Detected by Lectin Typing

A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cristigali, and Triticum vulgaris were used to type the strains. The lectins were chosen for their specificities for sugars commonly encountered in the lipopolysaccharide of H. pylori. The isolates were received from their parent institutions as pellets of biomass and were typed at one of three centers (in Ireland, Sweden, and Estonia). All 16 possible lectin reaction patterns were observed in the study, with the isolates with the predominant pattern exhibiting reactions with all the lectins in the panel. For European patients suffering from gastritis, an association was noted between lectin reaction pattern MH4 and atrophic chronic gastritis; isolates with lectin reaction pattern MH4 were isolated from patients with atrophic chronic gastritis, whereas isolates with this pattern were not isolated from patients with chronic gastritis (P = 0.0006). In addition, statistically significant relationships were noted between the lectin reaction pattern and the associated pathology of isolates from the Swedish population. Isolates with patterns MH13 and MH16, which had low lectin reactivities, correlated with nonulcer disease (P = 0.0025 and P = 0.0002, respectively), and all four isolates from adenocarcinoma patients were characterized as possessing reaction pattern MH16. In contrast, isolates with lectin reaction patterns MH1 and MH10, which had high lectin reactivities, were associated with ulcer disease (P = 0.046 and P = 0.0022, respectively)